The health benefits of sweet lupin seed flours and isolated proteins

The interest for lupin is continuously growing: one driving force are the numerous studies showing it provides useful health benefits. This review discusses available literature in the area of dyslipidaemia, diabetes, and hypertension prevention, providing hints on the mechanism of action. The addition of lupin protein to the diet of different models of hypercholesterolaemia, such as rat, rabbit, hamster and pig, induce decreases of total and non-HDL cholesterol. The clinical investigations on the cholesterol lowering activity provided controversial results. Those involving hypercholesterolaemic subjects and based on improved lupin foods gave statistically significant total and/or LDL-cholesterol reductions: both protein and fibre are relevant. The moderate hypotensive activity observed in some studies is probably linked to digestion-released ACE-inhibitory peptides. The hypoglycaemic activity, observed in post-prandial studies, is due to gamma-conglutin, a specific protein fraction. All this information suggests that lupin seeds may become a source of ingredients of innovative functional foods

Pediatric Response to Second-Line Antiretroviral Therapy in South Africa

Background: With improved access to pediatric antiretroviral therapy (ART) in resource-limited settings, more children could experience first-line ART treatment failure. Methods: We performed a retrospective cohort analysis using electronic medical records from HIV-infected children who initiated ART at McCord Hospital's Sinikithemba Clinic in KwaZulu-Natal, South Africa, from August 2003 to December 2010. We analyzed all records from children who began second-line ART due to first-line treatment failure. We used logistic regression to compare viral outcomes in Protease Inhibitor (PI)-based versus Non-Nucleoside Reverse Transcriptase Inhibitor (NNRTI)-based second-line ART, controlling for time on first-line ART, sex, and whether HIV genotyping guided the regimen change. Results: Of the 880 children who initiated ART during this time period, 80 (9.1%) switched to second-line ART due to therapeutic failure of first-line ART after a median of 95 weeks (IQR 65–147 weeks). Eight (10%) of the failures received NNRTI-based second-line ART, all of whom failed a PI-based first-line regimen. Seventy (87.5%) received PI-based second-line ART, all of whom failed a NNRTI-based first-line regimen. Two children (2.5%) received non-standard dual therapy as second-line ART. Six months after switching ART regimens, the viral suppression rate was significantly higher in the PI group (82%) than in the NNRTI group (29%; p = 0.003). Forty-one children (51%) were tested for genotypic resistance prior to switching to second-line ART. There was no significant difference in six month viral suppression (p = 0.38) between children with and without genotype testing. Conclusion: NNRTI-based second-line ART carries a high risk of virologic failure compared to PI-based second-line ART

STUDIO DELL'INTERAZIONE TRA LE PROTEINE CITOSCHELETRICHE 4.1R80 E 4.1R135 E LA PROTEINA ICLN: ASPETTI MOLECOLARI E FUNZIONALI.

ICln is a multifunctional protein that has been implicated in many cellular functions, from cell volume regulation to RNA metabolism. It interacts with many members or regulators of the cytoskeleton, like the 4.1R protein, actin, myosin light chain and the JBP1 protein. This suggests that the cytoskeleton rearrangement that follows an hypotonic challenge could be involved in the translocation of the ICln protein from the cytosol to the cell membrane and in the activation of the ICl,swell. In this work we tried to elucidate the role of ICln-4.1R interaction in the context of Regulatory Volume Decrease; we focused on the in vivo interaction between ICln and the protein 4.1R by the use of the FRET technique. We used two different 4.1R isoforms for the FRET experiments: an high molecular weight (HMW) isoform (4.1 long) and a low molecular weight (LMW) one (4.1 short, without the initial 209 aa box). The strongest FRET signal was measured between ICln and 4.1Rsh, and it was significally increased by an hypotonic shock. Moreover, when the two proteins are co-expressed and interact, it seems that the two proteins change each other localization showing a decreased nuclear and, for 4.1R, membrane localization. From a functional point of view, preliminary patch-clamp experiments suggest that the over-expression of the 4.1sh isoform in HEK cells leads to an increase of the ICl,swell (the anion current that allows RVD) activation, thus confirming a role for 4.1R in cell volume regulation

Detection of Earth-like Planets Using Apodized Telescopes

The mission of NASA's Terrestrial Planet Finder (TPF) is to find Earth-like planets orbiting other stars and characterize the atmospheres of these planets using spectroscopy. Because of the enormous brightness ratio between the star and the reflected light from the planet, techniques must be found to reduce the brightness of the star. The current favorite approach to doing this is with interferometry: interfering the light from two or more separated telescopes with a $\pi$ phase shift, nulling out the starlight. While this technique can, in principle, achieve the required dynamic range, building a space interferometer that has the necessary characteristics poses immense technical difficulties. In this paper, we suggest a much simpler approach to achieving the required dynamic range. By simply adjusting the transmissive shape of a telescope aperture, the intensity in large regions around the stellar image can be reduced nearly to zero. This approach could lead to construction of a TPF using conventional technologies, requiring space optics on a much smaller scale than the current TPF approach.Comment: Accepted for publication in ApJ Letters, 9 pages, 6 figure

Gold and silver nanoparticle-based colorimetric sensors: New trends and applications

Gold and Silver nanoparticles (AuNPs and AgNPs) are perfect platforms for developing sensing colorimetric devices thanks to their high surface to volume ratio and distinctive optical properties, particularly sensitive to changes in the surrounding environment. These characteristics ensure high sensitivity in colorimetric devices. Au and Ag nanoparticles can be capped with suitable molecules that can act as specific analyte receptors, so highly selective sensors can be obtained. This review aims to highlight the principal strategies developed during the last decade concerning the preparation of Au and Ag nanoparticle-based colorimetric sensors, with particular attention to environmental and health monitoring applications

Frequency-domain nonlinear modeling approaches for power systems components - A comparison

Harmonic simulations play a key role in studying and predicting the impact of nonlinear devices on the power quality level of distribution grids. A frequency-domain approach allows higher computational efficiency, which has key importance as long as complex networks have to be studied. However, this requires proper frequency-domain behavioral models able to represent the nonlinear voltage-current relationship characterizing these devices. The Frequency Transfer Matrix (FTM) method is one of the most widespread frequency domain modeling approaches for power system applications. However, others suitable techniques have been developed in the last years, in particular the X-parameters approach, which comes from radiofrequency and microwave applications, and the simplified Volterra models under quasi-sinusoidal conditions, that have been specifically tailored for power system devices. In this paper FTM, X-parameters and simplified Volterra approaches are compared in representing the nonlinear voltage-current relationship of a bridge rectifier feeding an ohmic-capacitive dc load. Results show that the X-parameters model reaches good accuracy, which is slightly better than that achieved by the FTM and simplified Volterra models, but with a considerably larger set of coefficients. Simplified Volterra models under quasi-sinusoidal conditions allows an effective trade-off between accuracy and complexity

Effects of dislocation density on injection and temperature sensitivity of InGaN LED emission spectra: a combined experimental and simulation approach

The aim of this paper is to describe a combined simulation and characterization activity carried out on blue LEDs grown on templates with different threading dislocation densities (TDDs)